O-salicylaldehyde has been utilized for the N-term ser/thr selective ligation in organic condition, and we explored this chemistry in aqueous condition that led to discovery of N-term cys selectivity. (link)
Image-based host cell infection profiling using fluorescence superoxide probe perfectly discriminates 3 AI subtypes (H1N1, H5N9, H9N2). (link)
Live cell imaging of chloro-functionalized photo-affinity BODIPY probe reveals sub-cellular accumulations. This article was selected as cover feature. (link)
Cellular binding target of dansyl fluorophore containing vinylsulfone group was identified as 3-Phosphoglycerate dehydrogenase (PHGDH), a key enzyme in the biosynthesis of serine. (link)
Aggregation property of organic fluorophore can be a key for biomolecule sensing. We demonstrated bulk aggregation based fluorescence turn-on sensor for progesterone using Dansyl scaffold. It was selected as Hot Paper from Angew. Chem. (link)
Comprehensive evaluation of bio-orthogonal photo-crosslinker for bio-active small molecule was studied (BRD4 inhibitor case study). (link)
First 48 membered dansyl library (DS) synthesis and their photo-physical properties were reported. (link)
The first photo-affinity BODIPY scaffold was published in Chem. Comm. (link)
Transcriptome expression assisted non-directed proteomic profiling (TEAnDPP) strategy demonstrated identification of host cell response factors. (link)
Unbiased fluorophore tagging together with competitive chemical proteomic profiling reveals host cell cycle regulator from Salmonella. (link)
Novel method to distinguish pathogenic bacteria using proteome reactivity profile was published in Chem. Comm. (link)
Synthetic chemist point of view regarding activity-based protein profiling (ABPP) was published in BMB Reports. (link)
The first fluorescence image-based phenomic profiling study was published in PLOS ONE. (link)
The first BODIPY library paper was selected as JACS Select collection focused on Supramolecular and Chemical Cascade Approaches to Molecular Sensing ( J. Am. Chem. Soc. 2009, 131, 10077-10082).
Post-doctoral position: we are seeking for highly motivated applicant who has doctoral degree with experience on biochemistry, chemical biology, or molecular biology. Please submit application with CV and email address of references to Dr. Lee (junseoklee at chembiol.re.kr).
Graduate/Undergraduate/high school student: Highly motivated students are always welcome. Please send an email to Dr. Lee with a transcript and a letter describing yourself and academic interest.